The commercial success of biopharmaceuticals has resulted in the production requirement of metric tons of monoclonal antibodies (MAbs). Higher titers in the cell lines producing the MAbs (>10g/L) has placed huge demands on the downstream processing for these products. Higher efficiency and lower cost methods are needed in bioseparation technology.

Answering the need for lower cost, higher efficiency media used in the production of monoclonal antibodies, PolyBatics has developed PolyBindZ, a disposable replacement for agarose immobilized protein A. PolyBatics has created beads displaying multiple copies of the IgG Fc binding zz domain of protein A, suitable as a protein A:agarose replacement. As a result of the high density, natively folded and functionally-oriented protein displayed on the surface of the bead, binding is more rapid than typical diffusive models and provides a higher per unit binding capacity. In addition, the beads are orders of magnitude smaller (100- 200 nm) than traditionally used agarose, providing a larger surface area for binding. A two-minute binding protocol that binds more than 90% of the available IgG has been demonstrated at lab scale.

Flexible Production System

The PolyBatics platform can be engineered to simultaneously display multiple functional proteins on the surface of the beads as demonstrated by the display of the zz domain combined with silica or gold binding. This ability to engineer combinations of binding domains provides for implementation of unique separation strategies.

Custom Prototyping

 Ligands can be custom tailored to increase the binding specificity, reducing the number of downstream steps required in production.